The effect of substrates on the protease activities of the gut primordium homogenate.

<p>(A) Inverted image of zymograms of the same homogenate with gelatin (1) and collagen I (2). Molecular masses (expressed in kDa) are indicated. (B) Densitometric analysis of the scanned bands. Adjusted volume (a.v.): proteinase activity represented the integrated intensity of all the pixels in the band, excluding the background. Results represent the mean±SE of 6 different zymograms corresponding to 3 zymograms from each of the 2 different homogenates per experimental condition (*–*, reliable differences between the compared values, p≤0.05).