MBC analysis.

<p>PBMC were fixed and stained with B cell phenotyping markers (CD19, CD27 and IgD) and analyzed for B cell subsets by flow cytometry. B cell subset analysis was performed as shown in (A) (representative <i>S. haematobium-</i>uninfected child). Proportion of CD19-gated cells that were CD27⁺IgD⁻ (B, switched MBC), CD27⁺IgD⁺ (C, non-switched MBC), CD27⁻IgD⁻ (D, double negative MBC), and CD27⁻IgD⁺ (E, naive B cells) were determined for <i>S. haematobium</i>-infected and uninfected children at baseline and follow-up. (B, C, D, E) Horizontal bars represent median. Number of donors in each group: baseline <i>S.h.</i> −ve n = 19, baseline <i>S.h.</i> +ve n = 21, follow-up <i>S.h.</i> −ve n = 8 and follow-up <i>S.h.</i> +ve n = 7.