Expression of IgM, IgA and IgG on CD27⁺ and DN MBCs.

<p>PBMC were fixed and expression of IgM, IgA and IgG on CD27⁺ B cells was evaluated <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002094#pntd.0002094-Berkowska1" target="_blank">[46]</a>. Proportions of CD19⁺ gated cells that were IgM⁺IgD⁻CD27⁺ (A), IgA⁺IgM⁻IgD⁻CD27⁺ (B), and IgG⁺IgM⁻IgD⁻CD27⁺ (C) were determined for <i>S. haematobium-</i>infected and uninfected children at baseline. For immunoglobulin expression on DN MBCs, PBMC were fixed and stained with B cell subset markers (CD19, IgD and CD27) and DN MBCs measured for IgM, IgA and IgG expression. Proportion of CD19⁺CD27⁻IgD⁻-gated cells that were IgM⁺ (D), IgA⁺ (E) and IgG⁺ (F) were determined for <i>S. haematobium-</i>infected and uninfected children at baseline. Horizontal bars represent median. Number of donors in each group: <i>S.h.</i> −ve n = 8 and <i>S.h.</i> +ve n = 7.