<p>PBMC were fixed and stained with B cell phenotyping markers (CD19, CD21 and CD27) and analyzed for B cell subsets by flow cytometry. B cell subset analysis was performed as shown in (A) (representative <i>S. haematobium-</i>uninfected child). Proportion of CD19-gated cells that were CD27<sup>+</sup>CD21<sup>−</sup> (B, activated MBC), CD27<sup>+</sup>CD21<sup>+</sup> (C, classical MBC), CD27<sup>−</sup>CD21<sup>−</sup> (D, atypical MBC), and CD27<sup>−</sup>CD21<sup>+</sup> (E, naive B cells) were determined for <i>S. haematobium-</i>infected and uninfected children at baseline and follow-up. (B, C, D, E) Horizontal bars represent median. Number of donors in each group: baseline <i>S.h.</i> −ve n = 19, baseline <i>S.h.</i> +ve n = 20, fo...
<p>(<b>A</b>) Representative analysis of PBMCs from infected and control subjects. Lymphocytes were ...
<p>Flow cytometry analysis was performed to determine proportions and subsequently calculate absolut...
<p>Representative dot plots (<b>A)</b> and the corresponding strategy (<b>B</b>) to identify on a ga...
<p>PBMC were fixed and stained with B cell phenotyping markers (CD19, CD27 and IgD) and analyzed for...
<p>The following B cell subsets were quantified by flow cytometry, as detailed in <a href="http://ww...
<p>PBMC were fixed and expression of IgM, IgA and IgG on CD27<sup>+</sup> B cells was evaluated <a h...
<p>PBMCs were collected from HSPN patients and healthy controls. Then, they were stained with PerCP-...
<p>a) Initial cell- inclusion and exclusion gating. b) The main B cell phenotypes from which cytokin...
Activated CD3+CD4+ and CD3+CD8+ T cell populations of PBMCs from BCG+ donors (solid bars) and BCG- d...
<p>The B cell subsets were determined by flow cytometry: naïve cells (CD19+CD10-CD21+CD27-), activat...
<p>Frequencies of CD19 total B cells, CD19+CD27+ memory B cells, CD19+CD27+IgM negative class switch...
<p>Mononuclear cells from donor and MM patient BM were stained with different antibodies to determin...
<p>Flow cytometry analysis of PMBC samples collected from children at visit one and visit four exami...
<p>PBMC were analyzed by flow cytometry. (<b>A</b>) Representative plots gated on live, CD14-CD3-CD4...
<p>We first gated on the live (DAPI<sup>−</sup>) lymphocytes and then collected 10,000 events of CD1...
<p>(<b>A</b>) Representative analysis of PBMCs from infected and control subjects. Lymphocytes were ...
<p>Flow cytometry analysis was performed to determine proportions and subsequently calculate absolut...
<p>Representative dot plots (<b>A)</b> and the corresponding strategy (<b>B</b>) to identify on a ga...
<p>PBMC were fixed and stained with B cell phenotyping markers (CD19, CD27 and IgD) and analyzed for...
<p>The following B cell subsets were quantified by flow cytometry, as detailed in <a href="http://ww...
<p>PBMC were fixed and expression of IgM, IgA and IgG on CD27<sup>+</sup> B cells was evaluated <a h...
<p>PBMCs were collected from HSPN patients and healthy controls. Then, they were stained with PerCP-...
<p>a) Initial cell- inclusion and exclusion gating. b) The main B cell phenotypes from which cytokin...
Activated CD3+CD4+ and CD3+CD8+ T cell populations of PBMCs from BCG+ donors (solid bars) and BCG- d...
<p>The B cell subsets were determined by flow cytometry: naïve cells (CD19+CD10-CD21+CD27-), activat...
<p>Frequencies of CD19 total B cells, CD19+CD27+ memory B cells, CD19+CD27+IgM negative class switch...
<p>Mononuclear cells from donor and MM patient BM were stained with different antibodies to determin...
<p>Flow cytometry analysis of PMBC samples collected from children at visit one and visit four exami...
<p>PBMC were analyzed by flow cytometry. (<b>A</b>) Representative plots gated on live, CD14-CD3-CD4...
<p>We first gated on the live (DAPI<sup>−</sup>) lymphocytes and then collected 10,000 events of CD1...
<p>(<b>A</b>) Representative analysis of PBMCs from infected and control subjects. Lymphocytes were ...
<p>Flow cytometry analysis was performed to determine proportions and subsequently calculate absolut...
<p>Representative dot plots (<b>A)</b> and the corresponding strategy (<b>B</b>) to identify on a ga...