hES cells cultured in NM23-H1-MM on Anti-MUC1* surfaces have a single cell cloning efficiency that is 15 times higher than the cloning efficiency of ES cells cultures in bFGF over MEFs.
- Benoit J. Smagghe (45223)
- Andrew K. Stewart (128590)
- Mark G. Carter (67629)
- Laura M. Shelton (386971)
- Kyle J. Bernier (386972)
- Eric J. Hartman (386973)
- Amy K. Calhoun (386974)
- Vasilios M. Hatziioannou (386975)
- Gabriele Lillacci (252128)
- Brian A. Kirk (386976)
- Brian A. DiNardo (386977)
- Kenneth S. Kosik (33884)
- Cynthia Bamdad (45793)
Publication date
March 2013
DOIAbstract
<p>a-d) Cloning efficiency of ES cells cultured in NM23-H1 media over anti-MUC1* antibody surfaces or cultured in bFGF over MEFs. Cells were plated at density of 1,000 (a and c) or 3,000 (b and d) cells/well and cultured for six days and formed colonies were stained with alkaline phosphatase. e) Stained colonies were counted and cloning efficiency was calculated. ES cells cultured in the NM23-H1 media over an anti-MUC1* antibody surface have a cloning efficiency of 21%.</p
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