Gene expression affected by <i>fnr</i> during growth at pH 5.5.

<p>RNA was isolated from anaerobic cultures of JLS1115 (W3110 <i>fnr</i>) (gray bars) grown to stationary phase at pH 5.5 in buffered LBK. qRT-PCR was used to measure the differential expression of mRNA levels for <i>cfa</i>, <i>gadB</i>, <i>hdeA katE</i>, <i>sdhC</i>, and <i>frdB</i> in the <i>fnr</i> mutant compared to the wild-type using primers listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056796#pone-0056796-t002" target="_blank">Table 2</a>. Positive values denote higher expression in the <i>fnr</i> mutant than in the wild-type and vice-versa. Error bars represent SEM, n = 3 (RNA from independent cultures). The expression profile for each gene was verified in triplicate.