Chondroitin 4<i>-O-</i>sulfate (C4S) immunoreactivity is reduced at five days after ARSB injection after moderate compression-injury.

<p>Immediately after spinal cord injury, one µl of ARSB (10 U/ml) was injected at the injury site (arrow) and 0.5 mm rostral and caudal to this site (arrowheads). After 5 days, the mice were perfused, and sagittal spinal cord sections were analyzed by immunofluorescence using an antibody specific for C4S. C4S immunoreactivity is higher at the injury site in the buffer treated control mice (<b>A</b>) versus ARSB treated mice (<b>B</b>). (<b>C</b>) and (<b>D</b>) are higher magnifications of the insets in (<b>A</b>) and (<b>B</b>), respectively. Diamidino-phenylindole (DAPI, blue) was used for nuclear staining and merged with C4S immunoreactivity (<b>C,D</b>). Arrows and arrowheads indicate the injury site and injection sites, respectively.