Downmodulation of N-cadherin expression and invasion abilities of H1650ER cell line.

<p>(A) N-cadherin expression in the H1650ER cells transfected with siRNAs targeting N-cadherin after 48 hours. Protein expression measured by flow cytometry, one representative experiment. (B) The ability of invasion evaluated after transfection for 48 hours, using a matrigel-coated chamber and 20% FCS as chemoattractant. Scale bars: 25 µm. (C) Changes in AKT and phospho-AKT kinase activity level upon N-cadherin siRNA silencing by Western blot.