PPARγ expression is down-regulated in 3T3-A212P cells.

<p>(A) Western blot using an anti-PPARγ antibody showing PPARγ protein level in 3T3-CON, 3T3-WT and 3T3-A212P cells at the indicated time points during adipocyte differentiation. β-actin was used as a loading control. (B) Expression levels of PPARγ, C/EBPα, aP2 and Srebp1c in 3T3-CON, 3T3-WT and 3T3-A212P cells at the indicated time points during differentiation were assessed by real-time qPCR. Values were expressed as fold changes by normalizing to the level in control cells at Day 0. β-actin expression was used as an internal control. Data are presented as mean ± SEM. N = 3 independent experiments, each measured in triplicates. *p<0.05, **p<0.01, and ***p<0.001 vs. 3T3-CON cells at the same time points. (C) Expression of PPARγ in 3T3-CON, 3T3-WT and 3T3-A212P cells at day 6 was assessed by immuno-fluorescent staining using the anti-PPARγ antibody. DAPI was used for nuclear staining. Scale bar = 100 µm.