Inhibition of microtubule polymerization does not influence the maintenance of Orbit localization on fusomes.

<p>Green is GFP-tubulin, red is mRFP-Orbit, and blue is DAPI staining. (A) An early spermatocyte cyst incubated without colchicine. Note the cytoplasmic microtubule structures and distinct Orbit localization on growing fusomes. (B) An early spermatocyte cyst treated with colchicine; a branched fusome structure is intact. (C, D) Microtubule degradation by colchicine treatment: (C), a metaphase I cell without colchicine treatment; (D), a meiotic cell treated with colchicine. Scale bar = 10 µm.