Visualization of the anatomy of a coronal mouse brain slice with fluorescent cell tracer.

<p>(7A) Fluorescent images are captured using a 10× objective lens. (7B) The fluorescent image is superimposed on a darkfield DIRI image. The superimposed image provides substantially more detail and anatomical context; the darkfield image depicts neuronal cell bodies and fibers while fluorescent label provides visualization of selected axonal projections. Acquisition time was 40 seconds for a 10× fluorescent image and 5 minutes for a 20× darkfield image.