Images of <i>Phaseolus vulgaris</i> leucoagglutinin (PHAL)-labeled neuronal pathways (green areas).

<p>(8A) Darkfield DIRI image collected using a 20× objective lens. (8B) Fluorescence of PHAL-labeled neuronal pathways in green with DAPI stained features in Blue (shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058344#pone-0058344-g008" target="_blank">figure 8B</a>) collected using a 10× objective lens and. (8C) A composite image of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058344#pone-0058344-g008" target="_blank">Figures 8A and 8B</a> providing improved clarity of localization of PHAL-labeled neuronal pathways. (i.e., medial geniculate complex, MG) and white matter (i.e., dorsal hippocampal commissure, dhc). DIRI also enhances the appearance of white matter through which PHAL-labeled axons travel (indicated by arrows). Abbrevations include cc: corpus callosum; CTX: cerebral cortex; dhc: dorsal hippocampal commissure; ENTl: lateral part of entorhinal cortex; MG: medial geniculate complex; TEa: temporal association areas.