Cell cycle-regulated proteins from G1 to S and S to G2 detected by mass spectrometry.

<p>A) Comparison of the total number of proteins detected in this study (2,842 proteins) to two other studies of the HeLa cell proteome: Nagaraj et al., 2011 (10,237 proteins) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058456#pone.0058456-Nagaraj1" target="_blank">[39]</a> and Olsen et al., 2010 (6,695 proteins) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058456#pone.0058456-Olsen1" target="_blank">[8]</a>. B) Quantified proteins from this study were divided into lists based on their fold and direction of change; the total protein count for each list is plotted. “NC” denotes proteins that did not change. “NC MG,” “Inc MG,” and “Dec MG” denote proteins that either did not change, increased, or decreased in response to MG132 treatment, respectively. C) All quantifiable proteins in the G1 to S dataset plotted by their log₂ transformed isotope ratios (medium S phase/light G1 phase). Dotted lines denote the 1.5-fold change threshold. D) All quantifiable proteins identified in the S to G2 dataset plotted by their log₂ transformed isotope ratios (medium G2 phase/light S phase); dotted lines denote the 1.5-fold change threshold. E) Proteins identified in early-S phase cells compared to early-S phase cells treated with MG132 plotted by their log₂ transformed isotope ratios (heavy S phase plus MG132/medium S phase minus MG132). Dotted lines denote the 1.5-fold change threshold. F) Proteins identified in G2 phase cells compared to G2 phase cells treated with MG132 plotted by their log₂ transformed isotope ratios (heavy G2 plus MG132/medium G2 phase minus MG132). Dotted lines denote the 1.5-fold change threshold.