Mechanism of action of PLK1 inhibitor <i>in vitro</i> and <i>in vivo</i>.

<p><i>In vitro</i> experiments<a href="http://:" target="_blank">:</a> AML-NS8 cells were untreated (CTRL) or treated with 0.2 µM NMS-P937 for 24 hours or 0.75 ng/ml nocodazole for 16 hours. (<b>A</b>) biparametric analysis for phospho-Histone H3 and DNA content by flow cytometry. Inserts show the correspondent cell cycle profile and indicate the percentage of cells in G2/M phase. (<b>B</b>) Western blot analysis of total cell lysates for the following proteins: phospho-NPM1 (pNPM1), NPM1, phospho-Histone H3 (pH3), Histone H3 (H3). Tubulin protein expression was used as loading control. <i>In vivo</i> experiments: (<b>C</b>) Solid tumour masses from vehicle or NMS-P937 treated animals were collected, fixed and stained with H&E or antibodies against phospho-TCTP (pTCTP), phospho-Histone H3 (pH3), phospho-NPM1 (pNPM1) or active Caspase 3. Representative pictures are reported (Axio Scope Zeiss, magnification×400). Black/white bar, 50 µm.