Nuclear and chloroplastic transcription is differentially regulated during prolonged darkness.

<p>qRT-PCR normalization factors were estimated by comparing the levels of the nuclear-encoded <i>DLST</i> transcript (A) or the chloroplast-encoded <i>atpB</i> transcript (B) in three biological replicas of dark-treated cells (D48) and cells re-exposed to WL for 0.5, 6 and 24 h to the levels found in CWL cells. Equal amounts of total RNA was used as starting material for the cDNA synthesis. Bars represent the average <i>DLST</i> or <i>atpB</i> normalization factor as calculated by REST2009 from two (A) or three (B) different qRT-PCR runs.