<i>Gli1</i> deletion prevents expansion of CD11b⁺CD11c⁺ myeloid cell subsets.

<p>A–B) Immunofluorescent detection of β-gal (green), α-SMA (red), and DAPI (blue) in sham- and <i>H. felis</i>-infected Gli1^+/LacZ mice. C–D) RT-qPCR analysis of CD11b and CD11c in 2- and 6-month infected mice. E) Bar graphs representing the percentages of CD45⁺MHCII⁺ myeloid cells per total gastric cell number in 6-month <i>H. felis</i>-infected WT versus Gli1^−/− mice. F) Flow cytometric analysis of CD11b and CD11c in 6-month infected WT and Gli1^−/− mice. Values on dot plot represent the percentages of cells (relative to total CD45⁺MHCII⁺ myeloid cell population) +/− SEM from N = 3 mice per group. G) Flow cytometry of CD11c+ cells within the CD11b⁺ stomach population (R1 gate from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058935#pone-0058935-g003" target="_blank">Figure 3F</a>) of 6-month <i>H. felis</i>-infected WT versus Gli1^−/− mice. H) Flow cytometric analysis of CD11b⁺ bone marrow cells in 6-month <i>H. felis</i>-infected WT and Gli1^−/− mice. I) Flow cytometry of CD11c⁺ cells within the CD11b⁺ bone marrow population (P1 gate from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058935#pone-0058935-g003" target="_blank">Figure 3H</a>) of 6-month <i>H. felis</i>-infected WT and Gli1^−/− mice. Open and closed bars denote uninfected and infected mice respectively. Error bars represent the mean +/− SEM. For RT-qPCR graphs, N = 5–10 mice per group. For flow sorting bar graph, N = 3 mice per group. Values on flow cytometric dot blots represent the mean +/− SEM from 3 mice per group. *p<0.05.