Curcumin alters global proteomics.

<p>Whole cell lysate was prepared by disrupting mid-log phase untreated and curcumin (400 μM) treated cultures using chilled glass beads. Protein extracts were prepared by precipitation and clean-up as mentioned in ‘Materials and methods’. A) Equal quantity of protein extracts were resolved by SDS-PAGE. B) 200 µg of total cellular proteins from untreated and curcumin-treated cultures was resolved by two-dimensional gel electrophoresis; first by isoelectric focusing (IEF) on a 7 cm long Immobiline^TM pH 3–10 DryStrip, followed by SDS-PAGE. The gel was stained with Coomassie Brilliant Blue R-250. C) Enlarged images of the upper left (Inset I), upper right (Inset II), lower left (Inset III), and lower right (Inset IV) regions of the two-dimensional gels containing proteins from untreated and curcumin-treated cells are shown. The differentially expressed spots are indicated by red circles.