Shh overexpression exacerbates <i>Helicobacter</i> gastritis.

<p>A) H&E staining of NTG, CMV-Shh^WT and CMV-Shh^F200H mouse stomachs. Arrows indicate regions of inflammatory cell infiltration and mucous neck cell expansion, SPEM. B) Polymorphonuclear (PMN) cell scoring in NTG and CMV-Shh^WT mice. C) H&E staining of 6-month infected CMV-Shh^WT stomach. D) High power H&E staining of herniated epithelial glands in the gastric submucosa in 6-month infected CMV-Shh^WT stomach. A dysplastic area is indicated. E) RT-qPCR analysis of IL-1β, TNF-α and IFN-γ in 6 month-infected CMV-Shh^WT versus NTG stomachs. F) Bar graphs representing flow cytometric analysis of CD11b⁺CD11c⁺ cells in 6-month infected NTG and CMV-Shh^WT stomachs. Positive cell numbers in infected CMV-Shh^WT mice are expressed as a percentage of cells present in infected NTG controls (set to a 100%). Error bars represent the mean +/− SEM. N = 3 mice per group. **p<0.01; *p<0.05.