The effects of glucose on Nampt gene and protein expression levels in human islets.

<p>Total RNA or protein was isolated from islets treated for 1 hour in 20 mM glucose. <b>A:</b> Nampt gene expression was upregulated in the presence of 20 mM glucose compared to control (2.2 mM glucose) by qRT-PCR. Each bar represents the mean fold change normalized to β-actin from five separate experiments.*P<0.05; student T test <b>B:</b> Nampt protein content did not increase in the presence of 20 mM compared to 2.2 mM glucose by western blot analysis. Shown is a representative blot from 6 separate experiments.