Reduced XpsE-ECFP foci abundance parallelled by secretion recovery in the secretion channel.

<p>(A) Immunoblot analysis of protein level of XpsD in cellular fraction (labeled as C) and secreted α-amylase in supernatant (labeled as S). The <i>xpsD</i>⁻ mutant (XC1753) supplemented with the XpsD(MH62), wild-type XpsD (XpsD) or empty vector (vector) was grown in liquid media containing L-arabinose at increasing concentrations. Arrowhead indicates XpsD; *, cross-reactive band. (B) Quantitative analysis of XpsE-ECFP foci abundance in various strains grown in media with increasing concentrations of L-arabinose. Data are mean foci counts per 60 cells from 3 independent fields. *** P<0.001.