Colocalization of chromosome-encoded XpsE-ECFP foci with those of secretin by confocal microscopy.

<p>The <i>xpsD</i>⁻ strain (XC1753) was supplemented with the partially functional XpsD(MH62) (the 1^st row) or secretion-proficient XpsD(MH64) (the 2^nd row) or the wild-type XpsD (the 3^rd row) or an empty vector (the 4^th row) and immunostained for XpsD::Myc with monoclonal antibody against Myc and fluorescence-labeled with Alexa Fluor 488. The XpsE-ECFP fluorescence is shown in green (left panels) and the immunostained XpsD::Myc in red (central panels). Shown in the last column are merged images (right panels). Scale bar, 2 µm.