Graphical depiction of the analysis of cell-induced forces.

<p>Note that the analysis is performed in three dimensions with 3D data, even though the depiction here appears two-dimensional. <b>Top Row</b>: an MDA-MD-231 cell stably expressing GFP-constitutively active Src (GFP-ca-Src). <b>Bottom Row</b>: an MDA-MD-231 cell stably expressing GFP-Src (GFP-wt-Src). <b>Left Column</b>: 2D max intensity projection. <i>Green</i>: fluorescent image of GFP-constitutively active Src in an MDA-MD-231 cell; <i>Cyan</i>: beads in the PAA gel beneath the cell. The collagen channel has been suppressed for clarity. <b>Middle Column</b>: Heat map of the reconstructed amplitude of 3D deformation of the gel due to forces exerted by the cell, as calculated from the gel stiffness and the changes in bead positions at this time-point relative to the reference time-point. <b>Right Column</b>: Heat map of the amplitude of the 3D stress field at the gel surface caused by forces propagated from the cell by fibrils of the collagen gel, calculated from the displacement field (middle). See Movies S6 and S7 for movies showing the analysis of the full time series.