Steviol inhibits forskolin-stimulated apical chloride current in a time-dependent manner.

<p>After permeabilization of the basolateral membrane with amphotericin B, MDCK cell monolayers were mounted in hemichambers filled with a chloride gradient buffer. 20 µM of forskolin was added to stimulate apical chloride current. (<b>A</b>) Representative tracings of chronic effect (pretreatment with steviol for 5 minutes, 2, 6, 12, and 24 h) were shown. At the end of the experiment, 50 µM of Gly-H101 was added. (<b>B</b>) Summary of the data for the chronic effect of steviol on apical chloride current in permeabilized MDCK cell monolayers (mean±SE; <i>n</i> = 5−11 separate experiments; <i>**P</i><0.01 compared with controls)