RBPL-1 is conserved in <i>C. elegans</i>. a.

<p>Predicted structure of RBPL-1 (TAG-214). The conserved domains DWNN, zinc knuckle, and RING finger are indicated by blue, red, and gray boxes, respectively. The nuclear localization signal (NLS) is indicated by a green box. <b>b.</b> Protein sequence alignment highlights the conservation of the DWNN, Zn knuckle, and RING finger domains among human, rat, and <i>C. elegans</i> RBBP6 proteins. <b>c.</b> Full-length <i>rbpl-1</i> mRNA cloned by RT-PCR. <b>d.</b> RBPL-1 expressed in <i>E. coli</i>. Upper: SDS-PAGE analysis of the bacterially expressed GST-RBPL-1 fusion protein, indicated by the red arrow. Lower: western blotting analysis of GST-RBPL-1 fusion protein (the red arrow) using an anti-GST antibody.