The Δ3–8 mutation causes defects in chromosome localization of N-terminal cyclin B1 fragments.

<p><b>A</b>. The Δ3–8 mutation disrupts the chromosome association of cyclin B1 truncations in a context-dependent fashion. Representative images of mitotic BS-C-1 cells expressing mutated cyclin B1. White arrows indicate position of metaphase plate as seen in the DIC image. Scale bar = 10 µm. <b>B</b>. CER box-and-whisker plots for the constructs shown in part A. The red dotted line indicates the threshold of 1.6. N = 8–14, ** indicates p<0.01 and *** indicates p<0.001 compared to WT counterpart. Further details including mean CER, standard deviations, and Wilcoxon exact test p-values can be found in Table S2. <b>C</b>. Biochemical fractionation of HeLa cells stably expressing WT^1–41-GFP or Δ3–8^1–41-GFP. (1–41)-GFP was detected with a cyclin B1 antibody. (Abbreviations as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059169#pone-0059169-g001" target="_blank">Figure 1</a>).