Priming enhances BMDMs responses to non-HAd, but not HAd, <i>Ft</i>.

<p>(A) Primary BMDMs and (B) immortalized BMDMs (IMC) from wild-type and TLR2^−/− C57BL/6 mice (2.5×10⁵ cells/well) were treated with IFN-γ (50 ng/well) for 16 h prior to infection with MHB- and BHI-grown <i>Ft</i> LVS at a MOI of 100. (C) In a separate set of experiments priming was achieved by incubation of cells with 4% autologous serum for 16 h prior to infection with <i>Ft</i> LVS. The autologous serum was recovered from <i>Ft</i> LVS-infected mice at day 6 p.i. For both sets of experiments, supernatants were collected 24 h p.i. and were assayed for the presence of cytokines by CBA. Results represent the mean ± SEM from three independent experiments. **<i>P</i><0.01, ***<i>P</i><0.001. (All results shown were subjected to One-way ANOVA with Bonferroni's Post-test).