Gene expressions of CD106⁺ and CD106⁻ CV-MSCs.

<p>(<b>A</b>) The gene expression profile of CD106⁺ and CD106⁻CV-MSCs were determined using Affymetrix oligoarrays and the scatter plot shows the gene expression level. Red plots indicate genes up regulated in CD106⁺CV-MSCs, and green plots indicate genes up regulated in CD106⁻CV-MSCs. (<b>B</b>) Relative mRNA expressions of COX-2, IL-1α, IL-1β, IL-6 and IL-8 in CD106⁺ and CD106⁻CV-MSCs were determined by real-time PCR. (<b>C</b>) 10⁵ of CD106⁺ or CD106⁻ CV-MSCs were cultured for 72 hours and PGE₂ concentration in supernatant was measured by ELISA. (<b>D</b>) CD106⁺ or CD106⁻ CV-MSCs were cultured with IFN-γ for 24 hours and relative mRNA expressions of IDO1 were determined by real-time PCR. The results are presented as means ± SEM of three separate experiments.