N-terminal amino acid sequence and critical regions in the 5’-region of zebrafish <i>prmt8</i>.

<p>(A) Comparison of the N-terminal amino acid sequences of human and zebrafish Prmt1 and Prmt8. The N-terminal amino acid sequences of human PRMT1 (H1; NP_938074.2), zebrafish Prmt1 (Z1; NP_956944.2), human PRMT8 (H8; NP_956944.2) and zebrafish Prmt8 (Z8; Q5RGQ2.2) were aligned by COBALT (<a href="http://www.ncbi.nlm.nih.gov/tools/cobalt/" target="_blank">http://www.ncbi.nlm.nih.gov/tools/cobalt/</a>). The amino acids encoded by different exons are marked by boxes. The grey shading shows identical amino acid sequence in the four entries. The conserved sequence in human and zebrafish PRMT8 before the PRMT1/PRMT8 conserved region are underlined. The glutamine-rich and proline/histidine-rich sequences are shown in italics. The second glycyl residue that might be myristoylated, the second methionine residue that corresponds to the third and alternative initiating methionine proposed in rat PRMT8 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055221#pone.0055221-Kousaka1" target="_blank">[14]</a>, and the SGT at the conserved AdoMet-binding site that were mutated to AAA to make catalytic-inactive methyltransferase are highlighted. (<b>B</b>) Schematic representation of part of the zebrafish <i>prmt8</i> gene encompassing the 5’-flanking region, exon I to II including intron I. The accession number for zebrafish <i>prmt8</i> gene is Q5RGQ2.2. The region displayed comprises genome coordinates of the zebrafish chromosome 4 (in Jul. 2010 Zv9/danRer7 assembly). The potential NRSEs encompassed 21 nucleotides (10,230,373-10,230,353, and 10,229,127-10,229,107 complementary strand) were aligned by the consensus NRSE motif derived from the “core” motif (5′-NNCAGCACCNNGCACAGNNNC-3′). The red boxes show the NRSEs located within the 5′-flanking and the first intron of the <i>prmt8</i> gene. The MO1 and the MO2 binding site to pair with the <i>prmt8</i> initiating ATG and the second ATG are indicated.