Effect of genistein treatment and expression of miR-574-3p in PCa cells and specimens.

<p>(A) Expression of miR-574-3p in PCa cell lines (DU145 and PC3) and normal prostate epithelial cells (RWPE-1). Real-time PCR showed that the expression levels of miR-574-3p were down-regulated in PCa cell lines (DU145 and PC3). miR-574-3p expression was normalized to RNU48. Data are presented as mean ± SE. *, P<0.05. (B) Expression levels of miR-574-3p after treatment with genistein (25 µM and 50 µM). miR-574-3p expression increased by 30–50% in genistein treated cells compared with controls. *, P<0.05. (C) miR-574-3p expression in clinical samples (Adjacent normal tissue, n = 48; PCa, n = 48). miR-574-3p expression was determined by real-time PCR and normalized to RNU48. P<0.0001. (D) Real-time PCR showing correlation of clinicopathological characteristics with miR-574-3p expression.