Wnt production, secretion and stability.

<p>A) Anti-WNT3A western blots comparing the levels of mWNT3A produced by L cells or iCHO cells in cell lysates and conditioned media. B) Coomassie stain of purified hWNT3A and mWNT3A separated by SDS-PAGE. C) 293A-STF cells were treated with 200 ng/mL purified hWNT3A or mWnt3A protein that was either fresh from 4°C, or pre-incubated at 37°C for 6 hr or 24 hr.