Positive staining for exosome markers on nanoparticles isolated from uterine fluid and associated mucus.

<p>Exosome markers CD63 (A,E) and CD81(B,F) positively identify exosomes in the 100,000 g pellet from (a) uterine fluid (A–D) and (b) in the 100,000 g pellet following dissociation of mucus (E–H). All exosomes are bound to latex beads. Merged staining co-localises these markers (C, G) Negative controls have normal IgG replacing primary antibody (D, H). n = 5 separate samples examined for each of fluid and mucus.