NOA36 is located in the nucleoli of mammalian cells.

<p>A) Indirect immunofluorescence labelling of HeLa cells with an anti-NOA36 polyclonal antibody (in green). B), C) and D) Transfection assays with the construct p-Flag-NOA36 in HeLa cells (labelled in green with an anti-Flag monoclonal antibody). In A) and B) chromatin localization (blue) and merged images are also shown in all the assays. In C) an D), images taken with differential interference contrast (upper panel), along with the anti-Flag staining (in green, central panel). A merge image of both pictures has been added to facilitate a better estimation of the recombinant Flag-NOA36 distribution in cell population (lower panel). C) Several transfected cells in which the Flag-NOA36 nucleolar distribution (in green, central panel) can be evaluated. D) Flag-NOA36 can also be found at cytoplasm of 12.5% either as a diffuse staining or in a punctuated pattern showed in D. Bars: 10 µm.