Standard curves of linear plasmid spiked with or without an environmental sample.

<p>Standard curves of linear target plasmid containing the 28S rDNA spiked with DNA from the environmental sample (Maizuru100904) using various <i>Ostreopsis</i> species-specific primer and probe sets (A: <i>O</i>. cf. <i>ovata</i>; B: <i>Ostreopsis</i> sp. 1; C: <i>Ostreopsis</i> sp. 5; D: <i>Ostreopsis</i> sp. 6) were constructed with a 10-fold dilution series of the plasmid. Error bars represent standard deviation of triplicate PCR reactions. Black circle: plasmid containing the 28S rDNA spiked with DNA from the environmental sample. Open circle: plasmid containing the 28S rDNA without DNA from the environmental sample.