Genome Wide Transcriptional Profile Analysis of <em>Vitis amurensis</em> and <em>Vitis vinifera</em> in Response to Cold Stress

<div><p>Grape is one of the most important fruit crops worldwide. The suitable geographical locations and productivity of grapes are largely limited by temperature. <i>Vitis amurensis</i> is a wild grapevine species with remarkable cold-tolerance, exceeding that of <i>Vitis vinifera</i>, the dominant cultivated species of grapevine. However, the molecular mechanisms that contribute to the enhanced freezing tolerance of <i>V. amurensis</i> remain unknown. Here we used deep sequencing data from restriction endonuclease-generated cDNA fragments to evaluate the whole genome wide modification of transcriptome of <i>V. amurensis</i> under cold treatment. <i>Vitis vinifera</i> cv. Muscat of Hamburg was used as control to help investigate the distinctive features of <i>V. amruensis</i> in responding to cold stress. Approximately 9 million tags were sequenced from non-cold treatment (NCT) and cold treatment (CT) cDNA libraries in each species of grapevine sampled from shoot apices. Alignment of tags into <i>V. vinifera</i> cv. Pinot noir (PN40024) annotated genome identified over 15,000 transcripts in each library in <i>V. amruensis</i> and more than 16,000 in Muscat of Hamburg. Comparative analysis between NCT and CT libraries indicate that <i>V. amurensis</i> has fewer differential expressed genes (DEGs, 1314 transcripts) than Muscat of Hamburg (2307 transcripts) when exposed to cold stress. Common DEGs (408 transcripts) suggest that some genes provide fundamental roles during cold stress in grapes. The most robust DEGs (more than 20-fold change) also demonstrated significant differences between two kinds of grapevine, indicating that cold stress may trigger species specific pathways in <i>V. amurensis</i>. Functional categories of DEGs indicated that the proportion of up-regulated transcripts related to metabolism, transport, signal transduction and transcription were more abundant in <i>V. amurensis</i>. Several highly expressed transcripts that were found uniquely accumulated in <i>V. amurensis</i> are discussed in detail. This subset of unique candidate transcripts may contribute to the excellent cold-hardiness of <i>V. amurensis</i>.</p>