Deletion of maternal <i>Hdac2</i> leads to reduced CENP-A expression in mouse oocytes.

<p>(A) Immunocytochemical detection of CENP-A in WT, <i>Hdac2^−/−</i> and <i>Hdac1^−/+/Hdac2^−/−</i> full-grown oocytes; at least 20 oocytes from each genotype were analyzed, and the experiment was conducted 2 times. Shown are representative images and DNA was counterstained with Sytox green (green). The bar corresponds to 10 µm. The upper row shows an enlargement of the region in the outlined box. (B) Quantification of the data shown in panel A. Staining intensity of different proteins in WT eggs was set to 1 and the data are expressed as mean ± SEM. Signal intensities relative to WT in <i>Hdac2^−/−</i> and <i>Hdac1^−/+/Hdac2^−/−</i> are 81±4%, and 55±6% respectively. *, p<0.05; **, p<0.01.