Adoptive transfer of <i>in vivo</i> or <i>in vitro</i> activated CD8+ T cells and protection from influenza virus challenge.

<p>A) Ly5.1+ mice were immunized with LM-GP33 and then boosted with LCMV Arm. On day 8 post boost the mice were sacrificed and their spleens pooled. The CD8+ T cells were then isolated using MACS columns and 1.6×10⁶ or 0.8×10⁶ GP33+ CD8+ T cells were transferred into Ly5.2+ mice. A third group was given an equal volume of PBS. All three groups were then challenged with 3 LD₅₀ PR8-GP33 one day later. Weight loss after rechallenge was monitored until day 6 post rechallenge at which point the mice were sacrificed. Data are representative of at least 5 mice per group. B) Viral load in the lungs was analyzed on day 6 post challenge. C) Flow cytometric analysis of infiltrating GP33-specific CD8+ T cell in the lung was performed on day 6-post challenge. D) 20×10⁶, 10×10⁶, or 2×10⁶<i>in vitro</i> activated GP33-specific P14 CD8+ T cells were transferred into separate groups of mice, which were then challenged with 3 LD₅₀ PR8-GP33 the following day. The weight, and lung function of these mice was determined at different points following rechallenge. Viral loads were determined in the lungs at day 6 post rechallenge. Data are representative of at least 7 mice per group. Mice were anaesthetized using avertin. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003207#s2" target="_blank">Results</a> are representative of two independent experiments.