Validation rate and Misclassification rate of enhancers predicted using RFECS in H1 and IMR90.

<p>A.) Validation Rate in H1 measured by overlap with DNase-I HS, p300, NANOG, OCT4 or SOX2, B.) Misclassification Rate in H1 measured as overlap of UCSC TSS, C.) Validation Rate in IMR90 measured by overlap with DNase-I HS or p300, D.) Misclassification Rate in IMR90 measured as overlap of UCSC TSS, versus total number of enhancers determined by taking different enrichment cutoffs, are shown for all 24 modifications (red), predicted minimal set of H3K4me1/H3K4me2/H3K4me3 (green) and conventionally used marks H3K4me1/H3K4me3 (black) or H3K4me1/H3K4me3/H3K27ac (blue). E.) Comparison of average validation rates for enhancer predictions using all combinations of 3 histone modifications for 2 replicates of H1.