Comparative analysis of anthracyclines effects on DNA double strand breaks: phosphorylation of serine 139 of H2AX (γH2AX).

<p>Cells were treated with the anthracyclines for 2 hours. Panel A presents representative images of HL-1 cell nuclei stained for γH2AX. The nuclei were counterstained with DAPI to detect specific signals and images were collected through confocal microscopy. Light microscopy images were taken for the field analyzed. Panel B presents the absolute value of the average number of foci detected in each nucleus for each treatment. Experiment was performed in quadruplicate. Abbreviations list: controlsCtrl; epirubicinEpi; doxorubicinDox; non-pegylated liposomal-doxorubicinLDox. Results are reported as Mean±Standard Error.