MSCs cultures from control and malnourished animals induced to adipogenic differentiation.

<p>(A) Cell culture morphology of differentiated MSCs from control animals cultured in adipogenic media (x 100); (B) Oil red O positive cells on day 14 after adipogenic differentiation of control MSCs. The red parts are lipid-rich vacuoles, the blue ones are nuclei counterstained with the standard May-Grunwald Giemsa solutions (x 100); (C) Cell culture morphology of differentiated MSCs from malnourished animals cultured in adipogenic medium (x 100); (B) Oil red O positive cells on day 14 after adipogenic differentiation of malnourished MSCs. The red parts are lipid-rich vacuoles, the blue ones are nuclei counterstained with the standard May-Grunwald Giemsa solutions (x 100). Representative pictures from control (n = 6) and malnourished groups (n = 6). The results showing the graphic percentage of quantified adipocytes and the graphic Oil red quantification from control (n = 6) and malnourished (n = 6) animals are expressed as as the means with standard deviations. The number in brackets denotes the number of animals used in the experiment. *(p ≤ 0.05) indicates where there was a significant difference between the control group and the malnourished group.