Age associated changes in adipogenic or osteogenic miRNAs and their target genes in SVF cells before and after adipogenic differentiation.

<p>MiR-143 that regulates adipogenic differentiation and miR-204 that regulates osteogenic differentiation and their target genes, were measured in SVF cells isolated from 6 mo and 30 rats (n = 8/group) before and after adipocyte differentiation using mirVana assays. miR-143, <b><i>4A</i></b>; miR-204, <b><i>4B</i></b>; PPARg, <b><i>4C</i></b>; ap2, <b><i>4D</i></b>; and Runx2, <b><i>4E</i></b>. The RT-qPCR data was expressed as fold of relative expression±SEM (Standard Error of Mean). Preadipocytes from 6 mo rats were defined as control. *: p<0.05; **: p<0.01; ***: p<0.005.