Impaired protein expression of miR-143 target genes with aging in preadipocytes before and after adipogenic differentiation.

<p>A decreased adipogenic differentiation potential of preadipocytes from old rats (30 mo) compared to 6 mo (n = 8/group) rats was due to changes in miR-143 levels. Western blot was used to analyze the protein levels of miR-143 target genes, ERK5, <i>5A</i> and <i>B</i>; PPARg, <i>5A</i> and <i>C</i>; or osteogenic marker, Runx2, <i>5A and D</i> in the young and old rats preadipocytes before and after adipogenic differentiation. RT-qPCR was also used to measure adipogenic markers, adiponectin, <i>5E</i> and IL-6, <i>5F</i>. The data was expressed as ratio or fold of relative expression±SEM (Standard Error of Mean). *: p<0.05; **: p<0.01.