qRT-PCR expression analysis of antioxidant enzymes in cucumber seedlings and roots of four weeks old <i>Arabidopsis</i> under salt stress.

<p>(A) RNA was extracted from untreated <i>Arabidopsis</i> roots and from roots collected from plants exposed to 100 mM NaCl (NaCl), and from roots colonized by T203 for 48 hours prior exposure to 100 mM NaCl (T+NaCl). Hierarchical clustering by Euclidean distance method average linkage is shown. The color of the each cell indicates fold-change relative to control as follows: Red: significant up regulation (<i>P</i><0.05; <i>t</i> test), green: significant down regulation (<i>P</i><0.05; <i>t</i> test). Black: not statistically significant difference from the control,. (B) RNA was extracted from untreated cucumber seedlings (Control), from seedlings exposed to 100 mM NaCl (+NaCl), from seedlings colonized by T203 (+T) and from seedlings colonized by T203 for 48 hours prior exposure to 100 mM NaCl (+T+NaCl). RNA was extracted 1 day and 4 days after salt addition. Fold expression (for A and B) was calculated from average of three independent biological repetitions.