Un-induced lentiviral shRNA-transduced cell lines behave similarly to each other.

<p><b>a</b>) Relative cell proliferation measured by MTT assay (24–96 hr time points) of U251^Ng2x, U251^Rictor, U251^EGFR and U251^EGFR/Rictor in the absence of doxycycline. Optical density values are normalized to values obtained at 24 hrs. <b>b</b>) Fraction affected (normalized to untreated cells) measured by MTT assay of U251^Ng2x, U251^Rictor, U251^EGFR and U251^EGFR/Rictor in absence of doxycycline and treated with irinotecan, vincristine and temozolomide for 72 hrs. For <i>in vitro</i> data, all values shown represent the average from three independent experiments. <b>c</b>) Subcutaneous tumor weight 14 days after inoculation of U251^Ng2x, U251^Rictor, U251^EGFR and U251^EGFR/Rictor in the absence of doxycycline (3 mice/group).