PD-L1 and PD-L2 expression on dendritic cells regulates the iNKT cell cytokine secretions after IAV infection.

<p>Pulmonary DCs (10³) were isolated from lungs of influenza A infected PD-L1^−/−, PD-L2^−/− and wild type BALB/c mice and loaded with 1 µg of α-galactosylceramide for 3 hours and then cultured with negatively enriched iNKT cells (1×10⁴) from the spleen of naïve wild type mice. Supernatants were collected after 48 h and IL-4 and IFN- γ levels were measured by ELISA. Data presented as mean ± SEM. *** P>0.001 as calculated by a one-way ANOVA with Tukey post hoc test and is representative of four independent experiments with n of 5 per group.