Splenic PrP^Sc accumulation.

<p>Early PrP^Sc accumulation in spleens from naive mice (lane 1), mice immunized with FA alone (lane 2), agPrP alone (3) or agPrP+FA (4), 40 (A) or 80 (B) days after challenge. Splenic samples from 3 mice per group were enriched in PrP^Sc and blotted with the polyclonal antibody SAL 1 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059143#pone.0059143-Sachsamanoglou1" target="_blank">[39]</a>. Equal amounts of total protein from three mice per group were processed.