Induction of MUC1-specific CTL responses against PANC-1 cells by DC/tumor.

<p>(A) T cells (n = 3) were stimulated with the same number of fused cells that coexpressed both MUC1 and HLA-DR in eight types of DC/tumor in the same set of experiments. Stimulated T cells were incubated with PKH-26-labeled PANC-1 cells at a ratio of 80∶1 for cytotoxicity assays. (B) T cells (n = 3) stimulated by OPK/Mock or OPK/TGF-β were cocultured with PKH-26-labeled PANC-1 cells at the indicated E:T ratios. Percentage of cytotoxicity (mean ± SD) was determined by flow cytometry CTL assays. (C) T cells (HLA-A2⁺) (n = 5) stimulated by eight types of DC/tumor (HLA-A2⁺) (Imm/Mock, OK/Mock, PSK/Mock, OPK/Mock, Imm/TGF-β, OK/TGF-β, PSK/TGF-β, and OPK/TGF-β) were stained with FITC-conjugated mAbs against CD8 and PE-conjugated pentamer against HLA-A2/MUC1. The numbers of events in gated CD8⁺ T cells are shown. (D) The percentage of CD8⁺ T cells reactive to MUC1 among the whole CD8⁺ T cell population (n = 5) was shown as a percentage of the double-positive population (pentamer⁺CD8⁺) in the total CD8⁺ T cells. The results are expressed as the mean ± SD. ***<i>P</i><0.001; **<i>P</i><0.01; *<i>P</i><0.05.