<p>A) Representative immunoblot shows that inhibition of PI3K by Ly294002 decreases CNN1 expression, while inhibition of MEK by U0126 increases pAkt and CNN1 expression. Combined treatment with Ly294002 and U0126 partly reverses Ly294002 mediated Akt activation and CNN1 expression. B) Quantification of three independent immunoblotting experiments to show that sustained PI3K/Akt signaling is necessary for VSMC markers expression in hAoSMC in vitro, whereas MAPK/ERK signaling negatively regulates Akt activation and its induction of VSMC markers expression. C) Representative immunoblot shows that inhibition of PI3K/Akt signaling by Ly294002 abolishes Spry1 induced VSMC markers expression. D) Quantification of at least three independent immunob...
<p>ARPE19 cells were treated with 8 µM 7KCh for 24 hr and the mRNA inductions of the inflammatory ma...
Activation of the PI3K/Akt pathway is the causative mechanism for the suppression of breast cancer m...
<p>U87MG-SPHK1 and LN-382-SPHK1 cells were incubated with 50 µM LY294002 (A) or 5 µM SK-I (B) for 24...
<p>A) Representative immunoblot shows that overexpression of Spry1 increases VSMC marker CNN1, ACTA2...
<p>A) Representative immunoblot to verify overexpression of Spry1 increases, while overexpression of...
<p>Spry1 and Spry4 differentially regulate VSMC phenotypes in part by modulating different RTK signa...
<p>A) Co-immunofluorescence staining of Spry1, Spry4 and ACTA2 on mouse artery sections show that bo...
BACKGROUND: Changes in the vascular smooth muscle cell (VSMC) contractile phenotype occur in patholo...
<p>Wildtype (WT) and <i>Sprr3</i><sup>-/-</sup> (KO) VSMCs were not treated (NT) or treated with PI3...
<p>(A) Relative transcript levels of MMP2 were assessed in <i>Sprr3</i><sup><i>+/+</i></sup><i>ApoE<...
<p>Western blot and quantitative analysis of Total Akt (A), p-Akt (B), Bcl-2 (C), Bax (D), Cleaved c...
<p>(A) Representative immunoblotting depicting phosphorylated and total Akt expression at baseline o...
<p>(<b>A</b>) PI3K inhibitor LY294002 (LY) decreases doxorubicin efflux in cell lines 10B and A549. ...
<p>NRCMs were transfected with the indicated siRNA for 24 h and subsequently serum-starved for 24 h....
<div><p>(A) Quantitative RT-PCR analysis of both β-catenin and β2-MG transcripts in αT3-1 cells trea...
<p>ARPE19 cells were treated with 8 µM 7KCh for 24 hr and the mRNA inductions of the inflammatory ma...
Activation of the PI3K/Akt pathway is the causative mechanism for the suppression of breast cancer m...
<p>U87MG-SPHK1 and LN-382-SPHK1 cells were incubated with 50 µM LY294002 (A) or 5 µM SK-I (B) for 24...
<p>A) Representative immunoblot shows that overexpression of Spry1 increases VSMC marker CNN1, ACTA2...
<p>A) Representative immunoblot to verify overexpression of Spry1 increases, while overexpression of...
<p>Spry1 and Spry4 differentially regulate VSMC phenotypes in part by modulating different RTK signa...
<p>A) Co-immunofluorescence staining of Spry1, Spry4 and ACTA2 on mouse artery sections show that bo...
BACKGROUND: Changes in the vascular smooth muscle cell (VSMC) contractile phenotype occur in patholo...
<p>Wildtype (WT) and <i>Sprr3</i><sup>-/-</sup> (KO) VSMCs were not treated (NT) or treated with PI3...
<p>(A) Relative transcript levels of MMP2 were assessed in <i>Sprr3</i><sup><i>+/+</i></sup><i>ApoE<...
<p>Western blot and quantitative analysis of Total Akt (A), p-Akt (B), Bcl-2 (C), Bax (D), Cleaved c...
<p>(A) Representative immunoblotting depicting phosphorylated and total Akt expression at baseline o...
<p>(<b>A</b>) PI3K inhibitor LY294002 (LY) decreases doxorubicin efflux in cell lines 10B and A549. ...
<p>NRCMs were transfected with the indicated siRNA for 24 h and subsequently serum-starved for 24 h....
<div><p>(A) Quantitative RT-PCR analysis of both β-catenin and β2-MG transcripts in αT3-1 cells trea...
<p>ARPE19 cells were treated with 8 µM 7KCh for 24 hr and the mRNA inductions of the inflammatory ma...
Activation of the PI3K/Akt pathway is the causative mechanism for the suppression of breast cancer m...
<p>U87MG-SPHK1 and LN-382-SPHK1 cells were incubated with 50 µM LY294002 (A) or 5 µM SK-I (B) for 24...