Spry1 increases, Spry4 decreases <i>Myocd</i> expression associated with their corresponding regulation of PI3K/Akt signaling.

<p>A) Representative immunoblot to verify overexpression of Spry1 increases, while overexpression of Spry4 decreases pAkt and pFoxO3a. B) Quantification from at least three independent immunoblotting experiments shows Spry1 increases, while Spry4 decreases pAkt and pFoxO3a levels. C) Quantification of RT-qPCR from three independent experiments showing that overexpression of Spry1 increases, while overexpression of Spry4 decreases <i>Myocd</i> mRNA expression. D) Representative agarose gel analysis shows Spry1 increases, Spry4 decreases <i>Myocd</i> mRNA expression. E) Parallel experiment to C and D for immunoblotting assay to confirm overexpression of Spry1 increased pAkt, while overexpression of Spry4 decreased pAkt. F) Quantification of RT-qPCR from three independent experiments showing that knockdown of Spry1 decreases, while knockdown of Spry4 increases <i>Myocd</i> mRNA expression. G) Representative agarose gel analysis shows knockdown of Spry1 decreases, while knockdown of Spry4 increases <i>Myocd</i> mRNA expression. H) Parallel experiment to F and G for immunoblotting assay to verify knockdown of Spry1 decreased pAkt, while knockdown of Spry4 increased pAkt. Note: *: p<0.05, **: p<0.01.