Thermodynamic parameters for the association of alkaloids with tRNA from ITC at different temperatures^a.

a<p>All the data in this table are derived from ITC experiments conducted in CP buffer of 10 mM [Na⁺], pH 7.0 and are average of four determinations. <i>K_a</i> and Δ<i>H⁰</i> values were determined from ITC profiles fitting to Origin 7.0 software as described in the text. The values of Δ<i>G⁰</i> were determined using the equations and Δ<i>G⁰</i> = Δ<i>H⁰-T</i>Δ<i>S⁰</i>. Δ<i>Cp⁰</i> is the specific heat capacity and Δ<i>G^o_hyd</i> is the energy contribution from the hydrophobic transfer step. All the ITC profiles were fit to a model of single binding.