Experimental system for single molecule measurements of myosin VI.

Keigo Ikezaki (390850)
Tomotaka Komori (390851)
Toshio Yanagida (122322)

Publication date

March 2013

DOI

10.1371/journal.pone.0058912.g001

Abstract

<p>(A) A Qdot585-labeled myosin VI moving on an actin filament was illuminated using an evanescent field. Myosin VI was biotinylated via HaloTag at its N-terminus (motor domain) using biotin-Halo-ligand. A streptavidin conjugated Qdot585 (Life technologies) was attached to the motor domain of myosin VI using avidin-biotin interactions. (B) A typical stepping trace of myosin VI at 200 µM ATP. Examples of large, small and backward steps are indicated by the arrows.</p

Extracted data

We use cookies to provide a better user experience.

Data Protection

Experimental system for single molecule measurements of myosin VI.

Abstract

Extracted data

Experimental system for single molecule measurements of myosin VI.

Abstract

Extracted data

Related items

Related items